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phosphohsp27  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphohsp27
    Phosphohsp27, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphohsp27+antibody/Phospho-HSP27+(Ser82)+Antibody/pm38253602-166-26-40
    Average 94 stars, based on 288 article reviews
    phosphohsp27 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Tubular Cytoplasmic Expression of Zinc Finger Protein SNAI1 in Renal Transplant Biopsies: A Sign of Diseased Epithelial Phenotype?
    Article Snippet: The Envision system (DakoCytomation) was used for detection of the signals for these five antibodies. .. Moreover, serial sections (3 mm thick) of each of the four TRD biopsy specimens obtained from Patients 1 and 9 with chronic CNI-induced tubular toxicity and Patients 10 and 11 with chronic and/or acute CNI-induced tubular toxicity were incubated with SNAI1 antibody and with five different antibodies: monoclonal antibodies against HSP27 (Abcam) at a 1:50 dilution 1 hour at room temperature, phosphoHSP27 (Cell Signaling Technology, Danvers, MA) at a 1:50 dilution 1 hour at room temperature, p38-phosphoT180-Y182 (Abcam) at a 1:100 dilution 1 hour at room temperature, polyubiquitinylated conjugates (Enzo Life Sciences, Lausen, Switzerland) at a 1:10 dilution 1 hour at 3 372 15 November 2016 7:55 pm EO: AJP15_0667 14 15 16 17 18 19 20 Prunotto et al 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 room temperature, and rabbit polyclonal antibody against GSK3bephospho-S9 (Abcam) at a 1:20 dilution 1 hour at room temperature. .. The Envision system (DakoCytomation) was used for detection of the signals for these five antibodies.

    Bioprocessing:

    Article Title: Tubular Cytoplasmic Expression of Zinc Finger Protein SNAI1 in Renal Transplant Biopsies: A Sign of Diseased Epithelial Phenotype?
    Article Snippet: The Envision system (DakoCytomation) was used for detection of the signals for these five antibodies. .. Moreover, serial sections (3 mm thick) of each of the four TRD biopsy specimens obtained from Patients 1 and 9 with chronic CNI-induced tubular toxicity and Patients 10 and 11 with chronic and/or acute CNI-induced tubular toxicity were incubated with SNAI1 antibody and with five different antibodies: monoclonal antibodies against HSP27 (Abcam) at a 1:50 dilution 1 hour at room temperature, phosphoHSP27 (Cell Signaling Technology, Danvers, MA) at a 1:50 dilution 1 hour at room temperature, p38-phosphoT180-Y182 (Abcam) at a 1:100 dilution 1 hour at room temperature, polyubiquitinylated conjugates (Enzo Life Sciences, Lausen, Switzerland) at a 1:10 dilution 1 hour at 3 372 15 November 2016 7:55 pm EO: AJP15_0667 14 15 16 17 18 19 20 Prunotto et al 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 room temperature, and rabbit polyclonal antibody against GSK3bephospho-S9 (Abcam) at a 1:20 dilution 1 hour at room temperature. .. The Envision system (DakoCytomation) was used for detection of the signals for these five antibodies.



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    Fig. 7. Human phospho-kinase array identifies inhibition in EGFR, Hsp27 and c-Jun by DSG3 overexpression. (A) Human phospho-kinase array data presented as the log 2 ratio of fold change in H413-hDsg3.myc against Vect Ct. The cut-off values were between > 0.1 and < 0.1. A significant decrease in the phosphorylation of epidermal growth factor receptor (EGFR) Y1086, transcription factor AP-1 (c-Jun) S63 and heat shock protein beta-1 (Hsp27) <t>S78/S82</t> was observed among others shown (n = 1 biological sample and 2 technical duplicates). (B) Western blotting validation with the indicated antibodies and the ratio of phospho vs. total proteins are shown on the right (n = 2 biological replicates). Reduced expression of EGFR Y1086 in hDsg3.myc cells were also observed compared with Vect Ct (data not shown due to the high background).
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    Image Search Results


    Fig. 7. Human phospho-kinase array identifies inhibition in EGFR, Hsp27 and c-Jun by DSG3 overexpression. (A) Human phospho-kinase array data presented as the log 2 ratio of fold change in H413-hDsg3.myc against Vect Ct. The cut-off values were between > 0.1 and < 0.1. A significant decrease in the phosphorylation of epidermal growth factor receptor (EGFR) Y1086, transcription factor AP-1 (c-Jun) S63 and heat shock protein beta-1 (Hsp27) S78/S82 was observed among others shown (n = 1 biological sample and 2 technical duplicates). (B) Western blotting validation with the indicated antibodies and the ratio of phospho vs. total proteins are shown on the right (n = 2 biological replicates). Reduced expression of EGFR Y1086 in hDsg3.myc cells were also observed compared with Vect Ct (data not shown due to the high background).

    Journal: Molecular oncology

    Article Title: Desmoglein-3 induces YAP phosphorylation and inactivation during collective migration of oral carcinoma cells.

    doi: 10.1002/1878-0261.13177

    Figure Lengend Snippet: Fig. 7. Human phospho-kinase array identifies inhibition in EGFR, Hsp27 and c-Jun by DSG3 overexpression. (A) Human phospho-kinase array data presented as the log 2 ratio of fold change in H413-hDsg3.myc against Vect Ct. The cut-off values were between > 0.1 and < 0.1. A significant decrease in the phosphorylation of epidermal growth factor receptor (EGFR) Y1086, transcription factor AP-1 (c-Jun) S63 and heat shock protein beta-1 (Hsp27) S78/S82 was observed among others shown (n = 1 biological sample and 2 technical duplicates). (B) Western blotting validation with the indicated antibodies and the ratio of phospho vs. total proteins are shown on the right (n = 2 biological replicates). Reduced expression of EGFR Y1086 in hDsg3.myc cells were also observed compared with Vect Ct (data not shown due to the high background).

    Article Snippet: The following mouse and rabbit monoclonal/polyclonal antibodies (Abs) were used: D8H1X, rabbit Ab to YAP (14074; Cell Signalling Technology, Leiden, Netherlands); EP1675Y, rabbit Ab to YAP1 (phospho S127) (ab76252; Abcam, Cambridge, UK); D24E4, rabbit Ab to YAP/TAZ (8418; Cell Signalling Technology); LATS1/2, rabbit Ab (9153; Cell Signalling Technology); 5H10, mouse Ab against the N-terminus of Dsg3 (sc23912; Santa Cruz, Dallas, TX, USA); 33–3D, mouse IgM against Dsg2 (gift from Professor Garrod); PG 5.1, mouse Ab to Plakoglobin (65015; Progen, Heidelberg, Germany); H-300, rabbit Ab to Desmoplakin (sc-33555; Santa Cruz); 5C2, mouse Ab to PKP1 (Progen); PKP3, mouse Ab (ab151401; Abcam); HECD-1, mouse anti-N terminus of E-Cadherin (ab1416; Abcam); rabbit Ab to a-Catenin (C2081; Sigma); 6F9, mouse Ab to b-Catenin (C7082; Sigma); mouse Ab to K14 (gift from Professor Leigh); C-20, rabbit Ab to FAK (sc-558; Santa Cruz); Anti-phospho-FAK (pTyr397) rabbit Ab (SAB4504403; Sigma); D38B1, rabbit Ab to EGF Receptor (4267; Cell Signalling Technology); D7A5, rabbit Ab to PhosphoEGF Receptor (Y1068) (3777; Cell Signalling Technology); Phospho-EGFR (S695), rabbit Ab (PA5-104725; Thermo Fisher Scientific); Phospho-EGFR (Y1086), rat Ab (MAB89671; R&D Systems, Minneapolis, MN, USA); 60A8, rabbit Ab to c-Jun (9165; Cell Signalling Technology); 54B3, rabbit Ab to Phospho-c-Jun (S63) (2361; Cell Signalling Technology); G31, mouse Ab to HSP27 (2402; Cell Signalling Technology); PhosphoHSP27 (S78), rabbit Ab (2405; Cell Signalling Technology); Phospho-HSP27 (S82), rabbit Ab (2401; Cell Signalling Technology); Alexa Fluor 488 conjugated phalloidin for F-Actin (A12379; Thermo Fisher Scientific); C-20, rabbit Ab to FOXM1 (sc-502; Santa Cruz); H-432, rabbit Ab to Cyclin A (sc-751; Santa Cruz); PC10, mouse Ab to PCNA (sc-56; Santa Cruz); Y69, rabbit Ab to c-Myc (ab32072; Abcam); MIB-1, mouse Ab to Ki-67 (GA626; Agilent, Santa Clara, CA, USA); 14C10, rabbit Ab to GAPDH (2118; Cell Signalling Technology); B-6, mouse Ab to HSC 70 (sc-7298; Santa Cruz); Secondary Abs were anti-mouse/rabbit IgG peroxidase antibody produced in goat (A0168/A6667; Sigma); Alexa Fluor 488 goat anti-mouse/rabbit IgG (A11029/A11034; Thermo Fisher Scientific), and Alexa Fluor 568 goat anti-mouse/rabbit IgG (A11031/A11036; Thermo Fisher Scientific).

    Techniques: Inhibition, Over Expression, Phospho-proteomics, Western Blot, Biomarker Discovery, Expressing

    Fig. 8. Proposed model on how elevated DSG3 expression contributes to the suppression of YAP and its mediated collective cell migration. In OSCC cells, mutual exclusive regulation occurs between DSG3 and YAP. In cells with low DSG3, YAP is activated via EGFR-mediated signalling that involves phosphorylation of EGFR S695/Y1086 and downstream Hsp27 S78/S82 and c-Jun S63 and thereby, YAP nuclear localisation and gene transcription associated with cell migration involved FAK and p-FAK Y397. In contrast, in cells with high DSG3, EGFR signalling is inhibited by DSG3 that elicits YAP inhibition and phosphorylation. As a result, p-YAP S127 is recruited from the nucleus to the cytoplasm as well as towards the plasma membrane, leading to contact inhibition of cell locomotion and attenuated collective cell migration.

    Journal: Molecular oncology

    Article Title: Desmoglein-3 induces YAP phosphorylation and inactivation during collective migration of oral carcinoma cells.

    doi: 10.1002/1878-0261.13177

    Figure Lengend Snippet: Fig. 8. Proposed model on how elevated DSG3 expression contributes to the suppression of YAP and its mediated collective cell migration. In OSCC cells, mutual exclusive regulation occurs between DSG3 and YAP. In cells with low DSG3, YAP is activated via EGFR-mediated signalling that involves phosphorylation of EGFR S695/Y1086 and downstream Hsp27 S78/S82 and c-Jun S63 and thereby, YAP nuclear localisation and gene transcription associated with cell migration involved FAK and p-FAK Y397. In contrast, in cells with high DSG3, EGFR signalling is inhibited by DSG3 that elicits YAP inhibition and phosphorylation. As a result, p-YAP S127 is recruited from the nucleus to the cytoplasm as well as towards the plasma membrane, leading to contact inhibition of cell locomotion and attenuated collective cell migration.

    Article Snippet: The following mouse and rabbit monoclonal/polyclonal antibodies (Abs) were used: D8H1X, rabbit Ab to YAP (14074; Cell Signalling Technology, Leiden, Netherlands); EP1675Y, rabbit Ab to YAP1 (phospho S127) (ab76252; Abcam, Cambridge, UK); D24E4, rabbit Ab to YAP/TAZ (8418; Cell Signalling Technology); LATS1/2, rabbit Ab (9153; Cell Signalling Technology); 5H10, mouse Ab against the N-terminus of Dsg3 (sc23912; Santa Cruz, Dallas, TX, USA); 33–3D, mouse IgM against Dsg2 (gift from Professor Garrod); PG 5.1, mouse Ab to Plakoglobin (65015; Progen, Heidelberg, Germany); H-300, rabbit Ab to Desmoplakin (sc-33555; Santa Cruz); 5C2, mouse Ab to PKP1 (Progen); PKP3, mouse Ab (ab151401; Abcam); HECD-1, mouse anti-N terminus of E-Cadherin (ab1416; Abcam); rabbit Ab to a-Catenin (C2081; Sigma); 6F9, mouse Ab to b-Catenin (C7082; Sigma); mouse Ab to K14 (gift from Professor Leigh); C-20, rabbit Ab to FAK (sc-558; Santa Cruz); Anti-phospho-FAK (pTyr397) rabbit Ab (SAB4504403; Sigma); D38B1, rabbit Ab to EGF Receptor (4267; Cell Signalling Technology); D7A5, rabbit Ab to PhosphoEGF Receptor (Y1068) (3777; Cell Signalling Technology); Phospho-EGFR (S695), rabbit Ab (PA5-104725; Thermo Fisher Scientific); Phospho-EGFR (Y1086), rat Ab (MAB89671; R&D Systems, Minneapolis, MN, USA); 60A8, rabbit Ab to c-Jun (9165; Cell Signalling Technology); 54B3, rabbit Ab to Phospho-c-Jun (S63) (2361; Cell Signalling Technology); G31, mouse Ab to HSP27 (2402; Cell Signalling Technology); PhosphoHSP27 (S78), rabbit Ab (2405; Cell Signalling Technology); Phospho-HSP27 (S82), rabbit Ab (2401; Cell Signalling Technology); Alexa Fluor 488 conjugated phalloidin for F-Actin (A12379; Thermo Fisher Scientific); C-20, rabbit Ab to FOXM1 (sc-502; Santa Cruz); H-432, rabbit Ab to Cyclin A (sc-751; Santa Cruz); PC10, mouse Ab to PCNA (sc-56; Santa Cruz); Y69, rabbit Ab to c-Myc (ab32072; Abcam); MIB-1, mouse Ab to Ki-67 (GA626; Agilent, Santa Clara, CA, USA); 14C10, rabbit Ab to GAPDH (2118; Cell Signalling Technology); B-6, mouse Ab to HSC 70 (sc-7298; Santa Cruz); Secondary Abs were anti-mouse/rabbit IgG peroxidase antibody produced in goat (A0168/A6667; Sigma); Alexa Fluor 488 goat anti-mouse/rabbit IgG (A11029/A11034; Thermo Fisher Scientific), and Alexa Fluor 568 goat anti-mouse/rabbit IgG (A11031/A11036; Thermo Fisher Scientific).

    Techniques: Expressing, Migration, Phospho-proteomics, Inhibition, Clinical Proteomics, Membrane